Mass spectrometry of steroids
نویسندگان
چکیده
منابع مشابه
Systematic error in gas chromatography-mass spectrometry-based quantification of hydrolyzed urinary steroids.
Gas chromatography-mass spectrometry-based metabolite profiling can lead to an understanding of various disease mechanisms as well as to identifying new diagnostic biomarkers by comparing the metabolites related in quantification. However, the unexpected transformation of urinary steroids during enzymatic hydrolysis with Helix pomatia could result in an underestimation or overestimation of thei...
متن کاملDevelopment and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids.
BACKGROUND Congenital adrenal hyperplasia is a group of autosomal recessive disorders caused by a deficiency of 1 of 4 enzymes required for the synthesis of glucocorticoids, mineralocorticoids, and sex hormones. Analysis of 11-deoxycortisol (11DC), 17-hydroxyprogesterone (17OHP), 17-hydroxypregnenolone (17OHPr), and pregnenolone (Pr) in blood allows detection of these enzyme defects. METHODS ...
متن کاملt-Butyldimethylsilyl derivatives in the gas chromatography-mass spectrometry of steroids.
Hydroxylic compounds are usually converted into derivatives for analysis by g.1.c. and gas chromatography-mass spectrometry (g.1.c.-m.s.). Trimethylsilyl ethers have found the widest application, but other alkylsilyl derivatives (cf. Harvey & Paton, 1975) and halogenated analogues are of complementary value. Particular interest attaches to t-butyldimethylsilyl ethers ; they are more stable towa...
متن کاملIdentification and quantification of neutral fecal steroids by gas-liquid chromatography and mass spectrometry: studies
The excretion of fecal neutral steroids was studied in six normocholesterolemic subjects and one hypercholesterolemic subject fed a standardized diet supplemented with butter fat or corn oil. The neutral steroids in the diets and in feces were identified by gas-liquid chromatography combined with mass spectrometric analyses of the chromatographic effluents. The three main sterols in the diets w...
متن کاملIdentification of steroids in rat adrenal glands by liquid chromatography-thermospray mass spectrometry.
Liquid chromatography-thermospray mass spectrometry was applied to identify corticosterone, 11-deoxy-18-hydroxycorticosterone, 18-hydroxycorticosterone and aldosterone as the major corticosteroids in the intracellular and extracellular pools of rat adrenals.
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1977
ISSN: 0014-5793
DOI: 10.1016/0014-5793(77)80505-x